Cooper Medical School of Rowan University
Department of Biomedical Sciences
401 South Broadway
Camden, NJ 08103
University of Poona, Pune, India
CMSRU Excellence in Teaching Award 2018
Rowan Value Award for Student Centeredness 2017
CMSRU Excellence in Teaching Award 2015
Gastroenterology, Endocrinology, Helicobacter pylori, Irritable Bowel Syndrome, Gut microbiome, Cold shock, RNA chaperones, Toxin-antitoxin;active learning, medical education
I am carrying out research using the gut-microbiome-based precision medicine approach for two projects, (i) creation of evidence-based, customized treatment options for Helicobacter pylori infection and (ii) management of Irritable Bowel Syndrome (IBS)with respect to psychological disorders and dietary intake of high frucose corn syrup (HFCS). I also have been involved in research on cold shock response and adaptation of bacteria with special emphasis on RNA chaperone function of cold shock proteins as well as bacterial toxin antitoxin systems.
We are creating a globally applicable, evidenced-based algorithm to design precision medicine therapies for effective eradication of antibiotic resistant strains of Helicobacter pylori based on analyzed patient data in Camden. Designing and implementation of safe and effective, evidence-based eradication regimens will help to alleviate the economic burden of Helicobacter pylori-related diseases, and in particular, to reduce the incidence of peptic ulcer disease and gastric cancer. We are also creating fingerprints of gut microbiome for various IBS conditions with respect to HFCS consumption and psychological disorders. This will allow earlier identification of risk factors to help in the management of IBS.
We showed that the transcription antitermination activity of the CspA homologuesis essential for their critical function in cold acclimation of cells and this activity depends on their nucleic acid melting activity. These observations in Escherichia coli hold true for diverse organisms such as thermophilic bacteria and were used as guidelines and found to be truein higher systems such as plants by other research groups. We elucidated the mechanisms underlying the RNA chaperone function of Csps. Using detail DNA microarrayanalysis of cold shock response of E.coli cells and its cold-sensitive mutants, we discovered cellular targets of CspA homologues and other cold shockproteins such as IF1. A new class of rho-independent,Csp-responsive transcription terminators emerged from bioinformatics analysis of these data which shed more light on the cold-acclimation-essential RNAchaperone function of CspA homologues. We have developed biotechnological applications using various novel properties of Csps, for example, cold-shock vectors for protein production at low temperatures. My work on the bacterial toxin-antitoxin systems has led to the discovery of a TA-toxin with antitumor activity.
Rowan Inspira Health Hack Grant Challenge
Ronald Ikechi, Bradford D. Fischer, Joshua DeSipio and Sangita Phadtare. Irritable Bowel Syndrome: correlation to high fructose corn syrup consumption, management and biopsychosocial aspects. Healthcare. 2017; 26:5(2).
Maeda Y, Lin CY, Ishida Y, Inouye M, Yamaguchi Y, Phadtare S. Characterization of YjjJ toxin of Escherichia coli. FEMS Microbiol Lett. 2017; 364 (11).
Yamaguchi Y,Tokunaga N, Inouye M and Phadtare S. Characterization of LdrA protein of Escherichia coli. Journal of Molecular Microbiology and Biotechnology. 2014; 24:91-97.
Phadtare S. Escherichia coli cold-shock gene profiles in response to overexpression/deletion of CsdA, RNase R and PNPase and relevance to low-temperature RNA metabolism. Genes to Cells. 2012; 17:850-74.
Phadtare S. Unwinding activity of cold shock proteins and RNAmetabolism. RNA Biology. 2011; 8:394-734.
Awano N, Rajagopal V, Arbing M, Patel S, Hunt J, Inouye M and Phadtare S. Escherichia coliRNase R has dual activities, helicase and ribonuclease. Journal of Bacteriology.2010; 192:1344-52.
Awano N, Inouye M and Phadtare S. RNase activity of polynucleotide phosphorylase is critical at lowtemperature in Escherichia coli andis complemented by RNase II. Journal of Bacteriology. 2008; 190:5924-33.
Awano N, Xu C, Ke H, Inoue K, Inouye M and PhadtareS. Complementation analysis of the cold-sensitive phenotype of the Escherichia coli csdA deletion strain. Journalof Bacteriology. 2007; 189: 5808-15.
Phadtare S and Severinov K. Nucleic acid meltingby Escherichia coli CspE. Nucleic Acids Research. 2005; 33:5583-5590.
Inouye M and PhadtareS. Cold-shock response and adaptation at near-freezing temperature in micro-organisms.Science STKE. 2004; 237:(pe26).
Phadtare S, Tyagi S, Inouye M andSeverinov K. Three amino acids in Escherichiacoli CspE surface-exposed aromatic patch are critical for nucleic acidmelting activity leading to transcription antitermination and cold acclimationof cells. Journal of Biological Chemistry. 2002; 277:46706-46711.
Phadtare S, Inouye M and Severinov K.The nucleic acid melting activity of Escherichiacoli CspE is critical for transcription antitermination andcold-acclimation of cells. Journal of Biological Chemistry. 2002;277:7239-7245.
Joshua DeSipio, Susan Perlis, and Sangita Phadtare. One-a-Day Nutrition Questions to Enhance Learning and Retention of Nutrition Concepts for Medical Students. Medical Science Educator. August 2018.
DeSipio J, Gaughan J, Perlis S, Phadtare S. Use of Real Patients and Patient-Simulation-Based Methodologies for Teaching Gastroenterololgy to Pre-Clinical Medical Students. Healthcare. 2018 6(2).
Williams C, Perlis S, Gaughan J, Phadtare S. Creation and implementation of a flipped jigsaw activity to stilumate interest in biochemistry among medical students. Biochem Mol Biol Educ. May 2018.
Hector Lopez, Evan Goldman, John Gaughan and Sangita Phadtare. From anatomical knowledge to clinical comprehension: peer-oriented learning session to help medical students make the leap. Medical Science Educator. February 2017.
Joshua DeSipio and Sangita Phadtare. An Interactive Session on Nutritional Pathologies for Health Professional Students. Healthcare, in the special issue: The Close Relationship: Health and Nutrition 2015, 3, 519-528.
Phadtare S, Galt J and BrodskyB. Active learning approaches for nutrition education in the medical schoolcurriculum. Medical Science Educator. 2014; 24: 27-33.
Phadtare S, Abali E and Brodsky B. Over The Counter Drugs (and food supplement) exercise: A team based introduction to biochemistry for health professional students. Biochemistry andMolecular Biology Education. 2013; 41:384-387.